Crystallization and X-ray study of the artificial TBI protein, an experimental multiple-epitope vaccine against type 1 human immunodeficiency virus

The artificial protein TBI constructed from four T-cell epitopes and five n eutralizing B-cell epitopes is an experimental new-generation multiple-epit ope vaccine against human immunodeficiency virus (HIV-1). The TBI protein w as expressed in Escherichia coli cells, isolated, and purified to the homog eneous state. The growth conditions for protein crystal were found, and the crystals thus grown were studied by the method of X-ray diffraction analys is.