Improvement of gas chromatographic determination methods of volatile amines in fish and fishery products

Gas chromatographic determination methods of DMA (dimethylamine) and TMA (t rimethylamine) in fish and fishery products were improved. Analysis was bas ed on solvent extraction of amines from perchloric acid extract and subsequ ent gas chromatographic determination. In the first part possible formation of TMA during analysis using n-amyl alcohol as extractant was discussed. I n the second part a new developed determination method based on a capillary column in combination with tert-butyl methyl ether and isooctane, respecti vely, as solvents was presented. Peaks were very well separated and had nea rly no tailing. Limits of determination varied for MMA (monomethylamine), D MA and TMA between 0.01 and 0.9 mg amine-N/100g. Recovery rates varied betw een 91 and 106% and repeatabilities (RSD, n=6) between 1.5 and 7%, Using is ooctane as solvent additional volatile amines could be separated and determ ined.