Technical manual for manufacturing autologous fibrin tissue adhesive

PURPOSE: The aim of this article is to provide a concise and simple technic al manual for manufacturing autologous fibrin tissue adhesive derived from the precipitation of fibrinogen using a combination of ethanol and freezing for surgery. METHODS: AU materials and equipment needed to manufacture eth anol-based autologous fibrin tissue adhesive are Listed. In addition, step- by-step instructions are provided to allow for easy and rapid fibrin adhesi ve production. RESULTS: Ethanol-based autologous fibrin tissue adhesive can be manufactured in under 60 minutes. Furthermore, at our institution the s tartup cost for manufacturing ethanol-based autologous fibrin tissue adhesi ve was under $2,500.00. CONCLUSION: Ethanol-based autologous fibrin tissue adhesive is a safe, reliable, and easily manufactured autologous fibrin tis sue adhesive that can be made by a trained technician in any blood bank, ph armacy, or surgical laboratory.