The disease gene TSC1 has been genetically mapped to human chromosome
region 9q34, in a 4-cM interval between the markers D9S149 and D9S114.
Within this interval there is conflicting genetic evidence as to the
finer localization of the gene. We have used fingerprinting methods an
d hybridization to produce a 1.7-Mb overlapping clone map covering the
TSC1 candidate region, with a single gap of 20 kb. We have localized
12 previously cloned genes and 17 genetic markers on this map and have
confirmed the order of the genetic map. This deep set of overlapping
clones is now ready to be used for candidate gene isolation, for trans
cription studies, or for sequencing. (C) 1997 Academic Press.