Functions of the exosome in rRNA, snoRNA and snRNA synthesis

Citation
C. Allmang et al., Functions of the exosome in rRNA, snoRNA and snRNA synthesis, EMBO J, 18(19), 1999, pp. 5399-5410
Citations number
47
Categorie Soggetti
Molecular Biology & Genetics
Journal title
EMBO JOURNAL
ISSN journal
02614189 → ACNP
Volume
18
Issue
19
Year of publication
1999
Pages
5399 - 5410
Database
ISI
SICI code
0261-4189(19991001)18:19<5399:FOTEIR>2.0.ZU;2-S
Abstract
The yeast nuclear exosome contains multiple 3'-->5' exoribonucleases, raisi ng the question of why so many activities are present in the complex. All c omponents are required during the 3' processing of the 5.8S rRNA, together with the putative RNA helicase Dob1p/R/Mtr4p. During this processing three distinct steps can be resolved, and hand-over between different exonuclease s appears to occur at least twice. 3' processing of snoRNAs (small nucleola r RNAs) that are excised from polycistronic precursors or from mRNA introns is also a multi-step process that involves the exosome, with final trimmin g specifically dependent on the Rrp6p component. The spliceosomal U4 snRNA (small nuclear RNA) is synthesized from a 3' extended precursor that is cle aved by Rntlp at sites 135 and 169 nt downstream of the mature 3' end. This cleavage is followed by 3'-->5' processing of the pre-snRNA involving the exosome complex and Dob1p. The exosome, together with Rntlp, also participa tes in the 3' processing of the U1 and U5 snRNAs, We conclude that the exos ome is involved in the processing of many RNA substrates and that different components can have distinct functions.