Selective hydrolysis of nucleotides to nucleosides and free bases

The kinetics of the hydrolysis of 2'-deoxyadenosine-5'-monophosphoric acid (dAMP), 2'-deoxycytidine-5'-monophosphoric acid (dCMP), 2'-deoxyguanosine-5 '-monophosphoric acid (dGMP) and tymidine-5'-monophosphoric acid (dTMP) was studied in the presence of Xanthomonas maltophilia [1]. The reaction produ cts are nucleosides: 2'-deoxyadenosine (dA), 2'-deoxycytidine (dC), 2'-deox yguanosine (dG) and tymidine (dT), respectively, or the respective free bas es. Hydrolysis of dTMP and dGMP proceeded stepwise according to the sequenc e: nucleotide --> nucleoside --> free base, whereas no accumulation of the free base was observed during the hydrolysis of dAMP and dCMP. (C) 1999 Els evier Science S.A. All rights reserved.