J. Nakai et al., Evidence for a role of C-terminus in Ca2+ inactivation of skeletal muscle Ca2+ release channel (ryanodine receptor), FEBS LETTER, 459(2), 1999, pp. 154-158
Six chimeras of the skeletal muscle (RyR1) and cardiac muscle (RyR2) Ca2+ r
elease channels (ryanodine receptors) previously used to identify RyR1 dihy
dropyridine receptor interactions [Nakai et al, (1998) J, Biol, Chem, 273,
13303] were expressed in HEK293 cells to assess their Ca2+ dependence in [H
-3]ryanodine binding and single channel measurements. The results indicate
that the C-terminal one-fourth has a major role in Ca2+ activation and inac
tivation of RyR1. Further, our results show that replacement of RyR1 region
s with corresponding RyR2 regions can result in loss and/or reduction of [H
-3]ryanodine binding affinity while maintaining channel activity.