A resource of mapped human bacterial artificial chromosome clones

To date, despite the increasing number of genomic tools, there is no reposi tory of ordered human BAC clones that covers entire chromosomes. This proje ct presents a resource of mapped large DNA fragments that span eight human chromosomes at similar to 1-Mb resolution. These DNA fragments are bacteria l artificial chromosome (BAC) clones anchored to sequence tagged site (STS) markers. This clone collection, which currently contains 759 mapped clones , is useful in a wide range of applications from microarray-based gene mapp ing to identification of chromosomal mutations. In addition to the clones t hemselves, we describe a database, GenMapDB (http://genomics.med.upenn.edu/ genmapdb), that contains information about each clone in our collection.