Identification of amplified fragment length polymorphism markers associated with Karnal bunt (Neovossia indica) resistance in bread wheat

Amplified fragment length polymorphism markers were used to study polymorph ism and their association with Karnal bunt resistance genes in wheat (Triti cum aestivum L. emend. Fiori & Paoi.). Recombinant inbred lines developed f rom a cross of a Karnal bunt susceptible 'WL 711' and a Karnal-bunt resista nt line 'HD 29' were tested for resistance to 2 Karnal bunt isolates throug h artificial inoculations. Study of parents and a set of recombinant inbred lines (selected on the basis of Karnal bunt reaction) for amplified fragme nt length polymorphism with 6 primer combinations were analyzed and 336 loc i scored. Of these, 77 were polymorphic between the parents. Three of the p olymorphic markers were associated with Karnal bunt resistance genes.