Effect of eotaxin on the generation of reactive oxygen species from eosinophil cell line, YY-1

Background: The CC chemokine eotaxin is a selective chemoattractant for eos inophils. Eosinophils have been considered to be the major effector cells i n allergic inflammation, since not only eosinophil-specific granule protein s but also reactive oxygen species (ROS) from eosinophils may cause the dam age to the cells or tissue of the mucosal epithelium. In this study, we exa mined the effect of eotaxin on ROS from an eosinophil cell line, YY-1. Meth ods: ROS in luminol-dependent reaction were examined. Calcium ionophore A23 187 were added to the mixture of YY-1 cells with luminol, and then ROS were determined. Results: Eotaxin primed the production of ROS from YY-1 cells. ROS from untreated YY-1 cells evoked with calcium ionophore A23187 in lumi nol-dependent chemiluminescence gave a maximal value of 1,928 +/- 223 inten sity counts (IC; mean +/- SE, n = 4) and an integral value of 17.04 +/- 1.5 1 IC (x 10(-4)), while eosinophils that were treated with eotaxin gave a ma ximal value of 2,264 +/- 86 IC (10 nM), 2,691 +/- 124 IC (100 nM) and an in tegral value of 21.22 +/- 0.67 IC (x10(-4); 10 nM), 26.20 +/- 1.41 IC (x10- 4; 100 nM). Conclusion: Eotaxin might play important roles in the pathogene sis of allergic inflammation through eosinophil activation by priming of eo sinophil oxidative metabolism as well as involvement in selective eosinophi l chemotaxis.