Analysis of taxol and major taxoids in Himalayan yew, Taxus wallichiana

A reversed-phase column liquid chromatography method for the analysis of ta xol, 10-deacetylbaccatin III, baccatin IV, 1-hydroxybaccatin I, 2-acetoxybr evifoliol, brevifoliol, 2'-deacetoxydecinnamoyltaxinine J and 2'-deacetoxyt axinine J in yew needles has been developed using a Nova-Pak Phenyl column and a binary gradient profile. The various aspects of analysis such as extr action efficiency, detection limits, reproducibility and peak purity were v alidated using UV-Vis as well as photodiode array detection. (C) 1999 Elsev ier Science B.V. All rights reserved.