Comparison of the Quantiplex version 3.0 assay and a sensitized Amplicor Monitor assay for measurement of human immunodeficiency virus type 1 RNA levels in plasma samples

Citation
Hc. Highbarger et al., Comparison of the Quantiplex version 3.0 assay and a sensitized Amplicor Monitor assay for measurement of human immunodeficiency virus type 1 RNA levels in plasma samples, J CLIN MICR, 37(11), 1999, pp. 3612-3614
Citations number
11
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
37
Issue
11
Year of publication
1999
Pages
3612 - 3614
Database
ISI
SICI code
0095-1137(199911)37:11<3612:COTQV3>2.0.ZU;2-D
Abstract
This study evaluated correlation and agreement between version 3 of the Qua ntiplex human immunodeficiency virus type 1 (HIV-1) RNA assay (v3 branched DNA [bDNA]) and a sensitized Amplicor HIV-1 Monitor assay (reverse transcri ption [RT]-PCR) for the measurement of HIV RNA. Three hundred eighteen samp les from 59 randomly selected, HIV-1-seropositive persons on various drug p rotocols from the National Institute of Allergy and Infectious Diseases HIV outpatient clinic were studied. The results indicate that v3 bDNA and RT-P CR are highly correlated (r = 0.98) and are in good agreement (mean differe nce in log(10) copies/ml +/- 2 standard deviations = 0.072 +/- 0.371). The relationship between values obtained by both assays is given by the followi ng equation: log(10)v3 bDNA = -0.0915 + 1.0052.log(10)RT-PCR This represent s a 1.026-fold difference between log(10)RT-PCR values and log(10)v3 bDNA v alues.