Redox-cycling type electrochemiluminescence in aqueous medium. A new principle for the detection of proteins labeled with a ruthenium chelate

The first observation of electrochemiluminescence (ecl) based on the redox- cycling of a ruthenium chelate covalently linked to a protein and placed in aqueous medium containing neither solvent nor reducing compound is reporte d here. Ecl experiments based on redox-cycling were performed in phosphate buffer at carbon interdigitated microelectrode arrays. The light intensity represented 31% of that obtained using a non complexed Ru(bpy)(3)(2+) solut ion prepared in phosphate buffer containing proteins. (C) 1999 Elsevier Sci ence S.A. All rights reserved.