Indirect enzyme-linked immunosorbent assay for the identification of sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides)

Polyclonal antibodies produced against soluble muscle protein extracts from sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Pla tichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides) were used in an indirect enzyme-linked immunosorbent assay for the specific ide ntification of fillets from these flatfish species. The assay was performed in two different formats: microtiter plates and immunostick tubes. Immunor ecognition of antibodies adsorbed to their specific fish samples was made w ith goat antirabbit immunoglobulins conjugated to the enzyme horseradish pe roxidase. Subsequent enzymatic conversion of the substrate allowed unequivo cal identification of all flatfish species studied.