Long-term heart preservation using a new portable hypothermic perfusion apparatus

Objective: Perfusion storage is not often used clinically compared with sim ple immersion because of complicated circuits and demanding management. We developed a new apparatus for preservation combined with simple immersion a nd continuous coronary perfusion. Methods: The main characteristics of this apparatus are as follows: (1) hyp othermic storage, (2) does not require any energy source, (3) variable perf usion pressure, and (4) portability. The perfusion apparatus is composed of a storage chamber, a cooling chamber, and metal bars from which a perfusat e bag is suspended. Adult mongrel dogs were divided into two groups: the co ronary perfusion group (CP, n = 6) and the simple immersion group (SI, n = 6). Coronary vascular beds of the dog were washed out with a University of Wisconsin (UW) solution following cardiac arrest obtained using a GIK solut ion. The hearts were then excised. In the CP group, the heart graft, which was immersed in a 4 degrees C UW solution, was perfused with the same solut ion at a how rate of 35 similar to 50 ml/hr. In the SI group, the heart gra ft was immersed in a 4 degrees C UW solution only. The heart graft was pres erved for 12 hours in both groups. beta-adenosine triphosphate (beta-ATP), phosphocreatine (Pcr), and inorganic phosphate (Pi) levels were measured im mediately after excision of the heart, and at 3, 6, and 12 hours after pres ervation. beta-ATP, Pcr, and Pi values were expressed as a percentage of co ntrol values, which had been obtained immediately after excision of the hea rt. Water content of the myocardium was measured prior to and after 12-hour preservation. The preserved graft was then evaluated through orthotopic tr ansplantation. Results: beta-ATP/Pi levels at 6 and 12 hours after preservation were signi ficantly higher in the CP group than in the SI group (62 +/- 5 versus 39 +/ - 7%, 48 +/- 5 versus 22 +/- 8%, respectively, p < 0.05). Pcr/Pi levels at 6 and 12 hours after preservation were 30 +/- 9% and 22 +/- 8%, respectivel y in the CP group, while Pcr/Pi levels in the SI group were detected in onl y one case. There was no significant difference in water content either pri or to or after 12-hour preservation between the two groups. Histopathologic ally, irregular expansion and/or contraction of myocardial fibers were more severe in the SI group than in the CP group. The recovery rate of hemodyna mic parameters 2 hours after heart transplantation was significantly (p < 0 .05) higher in the CP group than in the SI group. Conclusion: Stable and safe long-term canine heart preservation with contin uous coronary perfusion associated with immersion is possible using this ne w apparatus, and may have broad clinical application.