Soluble HIV-1 infected macrophage secretory products mediate blockade of long-term potentiation: a mechanism for cognitive dysfunction in HIV-1-associated dementia
Hg. Xiong et al., Soluble HIV-1 infected macrophage secretory products mediate blockade of long-term potentiation: a mechanism for cognitive dysfunction in HIV-1-associated dementia, J NEUROVIRO, 5(5), 1999, pp. 519-528
It is generally accepted that viral and cellular products from immune compe
tent mononuclear phagocytes (MP) (brain macrophages and microglia) underlie
the neuropathogenesis of HIV-1-associated dementia (HAD), What remains una
nswered, however, is the composition of and mechanisms for such MP-induced
neurological dysfunctions. In attempts to address these issues culture flui
ds from HIV-1(ADA)-infected monocyte-derived macrophages (MDMs) (depleted o
r enriched with progeny virus) were placed onto the CA1 area of rat hippoca
mpal brain slices (the site of mammalian learning and memory) and neuronal
long-term potentiation (LTP) assayed. LTP was induced by high frequency sti
mulation (HFS). Lipopolysaccharide (LPS) served as a surrogate macrophage a
ctivator. Synaptic strength was assayed by the initial slope of evoked fiel
d excitatory postsynaptic potentials (EPSPs). Synaptic potentiation followi
ng HFS was observed in slices incubated with uninfected (control) MDM cultu
re fluids. The magnitude of the LTP response was 150.2 +/- 21.10% compared
to basal levels (n=6). Synaptic strength was enhanced in virus-infected (13
5.7 +/- 28.9%, n=8) and LPS-activated MDM (123.3 +/- 5.1%, n=7) but at lowe
r levels than controls. The lowest levels of LTP were in brain slices incub
ated with virus-infected and LPS-activated MDM fluids at (109.5 +/- 9.9% n=
12). Interestingly, bath application of progeny HIV-1 virions showed minima
l LTP effects. Virus-infected, LPS-activated MDM fluids, with progeny virus
, reduced synaptic strength but were not statistically different than repli
cate culture fluids depleted of virus. In contrast, IL-1 beta and quinolini
c acid, significantly diminished synaptic strength. These results, taken to
gether, suggest that soluble HIV-1-infected MDM secretory products, but not
virus per se, significantly affect LTP. This electrophysiological system,
which monitors neuronal function following cell exposure to HIV-1 infected
materials could provide a novel testing ground for therapeutics designed to
protect brain function in HAD.