Cytokine secretion of periodontal ligament fibroblasts derived from human deciduous teeth: effect of mechanical stress on the secretion of transforming growth factor-beta 1 and macrophage colony stimulating factor
S. Kimoto et al., Cytokine secretion of periodontal ligament fibroblasts derived from human deciduous teeth: effect of mechanical stress on the secretion of transforming growth factor-beta 1 and macrophage colony stimulating factor, J PERIOD RE, 34(5), 1999, pp. 235-243
The periodontal ligament may play an important role in tooth eruption, root
development and resorption. The tissue physiologically receives mechanical
force during mastication. We focused on the effects of intermittent mechan
ical strain on the cytokine synthesis of periodontal ligament (PDL) fibrobl
asts in vitro. The cells were derived from human periodontal ligament of de
ciduous teeth (HPLF-Y) and permanent teeth (HPLF). The two kinds of PDL cel
ls and human gingival fibroblasts (HGF) were cultured in flexible bottomed
culture plates. The cells were mechanically stretched at 5% elongation, 3-c
ycles/min for 24 h on d 7 in culture using a Flexercell(R) strain unit. Aft
er the stretching, we measured DNA content and alkaline phosphatase activit
y in the cell layer, transforming growth factor beta 1 (TGF-beta 1) and mac
rophage colony stimulating factor (M-CSF) contents in the conditioned mediu
m. The TGF-beta 1 level in the conditioned medium of HPLF was significantly
higher than that of HPLF-Y and HGF. It was stimulated by mechanical stretc
hing only on HPLF, whereas no significant effect was observed on HPLF-Y and
HGF. M-CSF secretion was inhibited by the stretching on all of HPLF, HPLF-
Y and HGF. 1 alpha,25 dihydroxy vitamin D-3 (D-3) Stimulated M-CSF secretio
n into the culture medium of both HPLF and HPLF-Y, but the stretching inhib
ited M-CSF secretion and completely blocked the enhancement by D-3 These da
ta suggest that periodontal ligament cells synthesize and secrete the molec
ules as autocrine or paracrine factors that affect bone remodelling and roo
t resorption and the level of those factors change in response to mechanica
l stress.