Re. Catalan et al., Endothelin-1 effect on tyrosine phosphorylation and on tyrosine phosphatase (PTP-1C) translocation in rabbit platelets, J RECEPT SI, 19(6), 1999, pp. 909-925
Citations number
37
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF RECEPTOR AND SIGNAL TRANSDUCTION RESEARCH
This study examined the temporal relationships of endothelin-1-stimulated r
abbit platelets tyrosine phosphorylated proteins. The effect of endothelin-
1 on tyrosine phosphorylation was dose- and time-dependent and caused rapid
tyrosine phosphorylation of three groups of proteins in the molecular mass
range 70-100 kDa, 100-150 kDa and 150-200 kDa. Significant protein tyrosin
e phosphatase activity and amount were found to be associated with the cyto
skeleton of endothelin-1-stimulated rabbit platelets. Under our experimenta
l conditions, translocation from the cytosolic fraction to the cytoskeleton
reached its highest levels within 10-20 sec of endothelin-1 stimulation. E
ndothelin-1-induced translocation of protein tyrosine phosphatase, associat
ed with the increase in its activity was demonstrated by immunoblotting and
immunoelectron microscopy.