Lectin binding sites in parotid acinar secretory granules of normal and isoproterenol treated rat

Citation
F. D'Amico et al., Lectin binding sites in parotid acinar secretory granules of normal and isoproterenol treated rat, J SUBMIC CY, 31(1), 1999, pp. 115-121
Citations number
47
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
JOURNAL OF SUBMICROSCOPIC CYTOLOGY AND PATHOLOGY
ISSN journal
11229497 → ACNP
Volume
31
Issue
1
Year of publication
1999
Pages
115 - 121
Database
ISI
SICI code
1122-9497(199901)31:1<115:LBSIPA>2.0.ZU;2-6
Abstract
Lectin staining patterns in secretory granules of rat parotid gland acinar cell of untreated and isoproterenol-injected animals were examined by elect ron microscopy. We used four lectin-gold complexes: Ulex europaeus agglutin in I (UEA-I), Helix pomatia agglutinin (HPA), wheat germ agglutinin (WGA), Glycine max agglutinin (SBA). Specimens were low temperature embedded in th e hydrophilic Lowicryl K4M resin. The normal acinar cells produced glycocon jugates which were positive for all of the lectins used and with a characte ristic topographic distribution in relation to the morphological type of gr anule. The cells of isoproterenol-treated rat showed marked ultrastructural changes in the size and structure of granules; significant changes in lect in binding sites in the granules were also observed.