Measurement for breath concentration of hydrogen and methane in horses

This study concerns the establishment of a simple testing method for breath concentration of hydrogen and methane in horses. Twenty-eight healthy thor oughbreds and 24 Arabians were used. Breath samples were collected using on e-minute closed circulatory respiration through an aluminum bag filled with 10 liters of pure oxygen, which was mounted on the subjects by means of a face mask. Breath samples obtained, were analyzed by gas chromatography. A significant correlation in both hydrogen and methane levels was observed fo r samples collected at separate times. These findings confirmed the usefuln ess of our approach for testing breath concentrations of hydrogen and metha ne in horses.