Effect of stobadine on cardiac injury induced by ischemia and reperfusion

The aim of this work was to evaluate the effect of the antioxidant stobadin e on ischemia/reperfusion-induced injury of the isolated rat heart. Experim ents were performed according to Langendorff. Ischemia was induced by stop- flow lasting 30 minutes and the duration of repefusion was 30 minutes. Repe rfusion of the ischemic heart induced dysrhythmias, with the most severe on es occurring in the first minutes of reperfusion. A significant increase in coronary perfusion pressure was observed starting after 15 min of reperfus ion. Stobadine (10(-6) M applied 3 minutes before onset of ischemia and dur ing reperfusion) prevented the deleterious effects to develop fully. The pr otective effect of stobadine observed in our experiments seems to be a cons equence of its antioxidant properties.