Stobadine and heart mitochondria

Previous work has shown, that stobadine-hydrochloride (-)cis-2,8-dimethyl-2 ,3,4,4a, 5,9b-hexahydro-14-pyrido(4,3b) indole administered in a single dos e 2 mg/kg of body weight reduces cardiotoxic effect of isoproterenol (1 mg/ kg) as shown by lowered serum enzyme activities of AST, CPK LDH and ALT. We studied the effect of stobadine in vivo on respiration, the level of ATP, malondialdehyde (MDA) and superoxiddismutase (SOD) in heart mitochondria. S erum enzyme activities of AST, CPK and LDH after stobadine application were significantly decreased. In mitochondria, respiration and activity of SOD were inhibited, level of MDA was increased and level of ATP was unchanged. The cardioprotective effect of stobadine is not linked to preservation of m itochondrial function. This effect is probably more complex and mediated on the level of the whole organism.