Effect of stobadine on lipid peroxidation in brain and heart after ischemia and reperfusion of the brain

Stobadine (ST), a novel drug with pyridoindol structure, was recently found to prevent reperfusion injury in rat brain. The aim of the present study w as to reveal whether ST may prevent peroxidative changes in the heart and b rain that were triggered by postischemic reperfusion of the brain. In the b rain, reperfusion significantly increased the contents of malondialdehyde ( MDA) by 43.8 % and conjugated diens (CD) by 24.5% when compared with the en d of ischemia. In the heart, contents of MDA and CD in reperfusion became e levated three fold and by 41.7 %, respectively, when comparing to the value s at the end of ischemia. In the heart, no significant changes in activitie s of the superoxide dismutase (SOD) and glutathione peroxidase (GPx) induce d by ischemia or reperfusion were detected. In contrast, reperfusion induce d a slight decrease in GPx activity in the brain. In accordance with our pr evious results, an application of ST (2 mg/kg) to the femoral artery shortl y prior to reperfusion of the ischemic brain, prevented significantly MDA a nd CD accumulation in brain. Nevertheless, ST was not able to prevent the b rain-ischemia/reperfusion-induced elevation of MDA and CD contents in the h eart.