Adjusted and unadjusted energy usage rates both determine body fat and plasma leptin in male Fischer 344 rats

Previous studies of the relationship between plasma leptin and energy usage have yielded contradictory findings. The present study was therefore condu cted to clearly distinguish and measure the energy usage rate and the energ y usage rate adjusted for a surrogate of metabolically active tissue mass. We investigated the simultaneous relationships between these two measures o f energy usage, leptin, and body fat in al-month-old adult male Fischer 344 rats on three different long-term dietary regimens: (1) continuous ad libi tum feeding (Ad-lib); (2) ad libitum feeding until early adulthood, and the n continuous 60% caloric restriction (CR); and (3) ad libitum feeding until early adulthood, then 60% caloric restriction until 16 months, and then ad libitum feeding for 5 months (CR/Ad-lib). Two versions of the daily usage rate were measured: daily dietary caloric intake (DCI), and daily energy ex penditure (EE) based on indirect calorimetry. Two versions of the metabolic ally active tissue mass were also measured: fat-free mass (FFM), and the su m of the weight of the heart, brain, liver, and kidneys. Energy usage rates were adjusted for these measures of metabolically active tissue mass to yi eld measures of the energy metabolic rate. Correlation, regression, and pat h analyses showed that both the energy usage rate and adjusted energy usage rate played important independent roles in determining body fat and plasma leptin, but only after multivariate techniques were used to account for th e simultaneous interactions between variables. Increases in the energy usag e rate were associated with increases in body fat and the adjusted energy u sage rate. Increases in the adjusted energy usage rate were associated with decreases in body fat and plasma leptin. These findings suggest that diffe rences in subjects adjusted energy usage rate could explain some of the app arently contradictory findings concerning the relationship between energy u sage and plasma leptin in previously published studies. In conclusion, this appears to be the first study to clearly separate and quantify the effects of the energy usage rate and adjusted energy usage rate on body fat and pl asma leptin. The findings suggest that under conditions of long-term stable body weight, both of these measures of energy usage play independent simul taneous roles in determining body fat and plasma leptin. Copyright (C) 1999 by W.B. Saunders Company.