EFFECT OF THE DEGREE OF SUBSTITUTION OF (2-HYDROXY)PROPYL-BETA-CYCLODEXTRIN ON THE ENANTIOSEPARATION OF ORGANIC-ACIDS BY CAPILLARY ELECTROPHORESIS

Optical isomers of nine organic acids were separated by high-performan ce capillary electrophoresis using (2-hydroxy)propyl-beta-cyclodextrin s, with a degree of the substitution between 3.0 and 7.3 (2-hydroxy)pr opyl groups/cyclodextrin molecule. The degree of substitution has a si gnificant influence on the resolution of the enantiomers and is theref ore an important tool in the optimisation of chiral separations. Accor dingly, a proper description of derivatized cyclodextrins should inclu de the degree of substitution.