NMR study on the sequence specificity of complex formation of the antibiotic daunomycin with isomeric tetradeoxynucleotides 5 '-d(ACGT), 5 '-d(AGCT),and 5 '-d(TGCA)

Authors
Eaton, RJ Veselkov, DA Pakhomov, VI Baranovskii, SF Bolotin, PA Osetrov, SG Djimant, LN Davies, DB Veselkov, AN
Citation
Rj. Eaton et al., NMR study on the sequence specificity of complex formation of the antibiotic daunomycin with isomeric tetradeoxynucleotides 5 '-d(ACGT), 5 '-d(AGCT),and 5 '-d(TGCA), MOL BIOL, 33(5), 1999, pp. 709-718
Citations number
42
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR BIOLOGY
ISSN journal
00268933 → ACNP
Volume
33
Issue
5
Year of publication
1999
Pages
709 - 718
Database
ISI
SICI code
0026-8933(199909/10)33:5<709:NSOTSS>2.0.ZU;2-W
Abstract
Formation of complexes of the antibiotic daunomycin with isomeric tetradeox ynucleotides 5'-d(ACGT), 5'-d(AGCT), and 5'-d(TGCA) was studied by one- and two-dimensional NMR spectroscopy in order to determine the sequence specif icity of ligand binding. The daunomycin chromophore orientation in complex with a tetradeoxynucleotide duplex in solution proved to substantially depe nd on the base sequence at the sites of antibiotic intercalation. At the sa me time, the characteristic structural features of intercalated daunomycin complexes with DNA fragments were retained for all the tetradeoxynucleotide s examined, regardless of the base sequence.