Synthesis of proteins with a variable number of anthracene-containing photoactive groups for structural analysis by luminescence techniques

The method was developed to attach a variable number of anthracene-containi ng photoactive groups to protein molecules. Active reagents 9-anthryl diazo methane and 9-anthrylmethyl isocyanate were allowed to react with carboxyl and amino groups of proteins, respectively, in a heterogeneous reaction. Wi th lysozyme and carboanhydrase B, a possibility was shown of studying the p rotein dynamics and structure with luminescence spectroscopy by varying the number of photoactive groups (on average, from 0.1 to 2.0) per protein mol ecule.