Association of the cell cycle transcription factor Mbp1 with the Skn7 response regulator in budding yeast

Citation
N. Bouquin et al., Association of the cell cycle transcription factor Mbp1 with the Skn7 response regulator in budding yeast, MOL BIOL CE, 10(10), 1999, pp. 3389-3400
Citations number
47
Categorie Soggetti
Cell & Developmental Biology
Journal title
MOLECULAR BIOLOGY OF THE CELL
ISSN journal
10591524 → ACNP
Volume
10
Issue
10
Year of publication
1999
Pages
3389 - 3400
Database
ISI
SICI code
1059-1524(199910)10:10<3389:AOTCCT>2.0.ZU;2-M
Abstract
We previously isolated the SKN7 gene in a screen designed to isolate new co mponents of the G1-S cell cycle transcription machinery in budding yeast. W e have now found that Skn7 associates with Mbp1, the DNA-binding component of the G1-S transcription factor DSC1/MBF. SKN7 and MBP1 show several genet ic interactions. Skn7 overexpression is lethal and is suppressed by a mutat ion in MBP1. Similarly, high overexpression of Mbp1 is lethal and can be su ppressed by skn7 mutations. SKN7 is also required for MBP1 function in a mu tant compromised for G1-specific transcription. Gel-retardation assays indi cate that Skn7 is not an integral part of MBF. However, a physical interact ion between Skn7 and Mbp1 was detected using two-hybrid assays and GST pull downs. Thus, Skn7 and Mbp1 seem to form a transcription factor independent of MBF. Genetic data suggest that this new transcription factor could be in volved in the bud-emergence process.