Autoradiographic evidence that intrastriatal administration of adenosine A(1) receptor antisense oligodeoxynucleotide decreases adenosine A(1) receptors in the rat striatum and cortex

In this study, the effect of a phosphorothioated A(1) adenosine receptor an tisense oligodeoxynucleotide on A(1) receptor density and mRNA in the stria tum and cortex of rats was determined. Receptor autoradiography and in situ hybridization revealed a reduction in striatal and cortical A, receptor de nsity and cortical A(1) receptor mRNA, respectively, in antisense-treated b rains but not in those treated with a mismatch oligonucleotide. There was n o change in A(1) receptor binding. These data imply that the corticostriata l pathway synthesizes A(2) receptors and transports them to its terminals. (C) 1999 Elsevier Science B.V. All rights reserved.