Ad. Mitchell et A. Bresinsky, Phylogenetic relationships of Agaricus species based on ITS-2 and 28S ribosomal DNA sequences, MYCOLOGIA, 91(5), 1999, pp. 811-819
Parsimony and maximum likelihood analyses of sequence data from the interna
l transcribed spacer (1TS-2) region plus a portion of the 288 gene are used
to study the phylogenetic relationships of Agaricus species. The ingroup c
ontained 16 Agaricus species, selected from the range of sections contained
in the subgenus Agaricus. A total of eight outgroups were chosen from the
Agaricaceae and phylogenetic trees rooted on Stropharia coronilla. Results
suggest that Agaricus may be monophyletic, but further tests should include
taxa from the subgenera Langagaricus and Conioagaricus. Two well-supported
clades were found. The Agaricus clade contained A. subperonatus, A. devoni
ensis, A. bisporus, A. spissicaulis, A. bitorquis, and A. impudicus. Result
s suggest that A. bernardii may be closely related to this group. The Arven
ses clade contained A. silvicola, A. arvensis, A. abruptibulbus, and A. sem
otus. A possible sister group to the Arvenses clade includes A. lanipes and
A. maskae. Relationships for A. campestris, A. silvaticus and A. xanthoder
ma remain unclear, although it is likely these species diverged early compa
red with the other Agaricus species studied. Phylogenetic hypotheses are gi
ven for taxa that have been difficult to classify based on other forms of d
ata, including the placement of A. spissicaulis with A. bisporus; A. lanipe
s with A. maskae; and Melanophyllum haematospermum with Leucoagaricus nauci
nus .