Quantum superposition lies at the heart of quantum mechanics and gives rise
to many of its paradoxes. Superposition of de Broglie matter waves(1) has
been observed for massive particles such as electrons(2), atoms and dimers(
3), small van der Waals clusters(4), and neutrons(5). But matter wave inter
ferometry with larger objects has remained experimentally challenging, desp
ite the development of powerful atom interferometric techniques for experim
ents in fundamental quantum mechanics, metrology and lithography(6). Here w
e report the observation of de Broglie wave interference of C-60 molecules
by diffraction at a material absorption grating. This molecule is the most
massive and complex object in which wave behaviour has been observed. Of pa
rticular interest is the fact that C-60 is almost a classical body, because
of its many excited internal degrees of freedom and their possible couplin
gs to the environment. Such couplings are essential for the appearance of d
ecoherence(7,8), suggesting that interference experiments with large molecu
les should facilitate detailed studies of this process.