The common cDNA and amino acid sequences of the CD14 (myeloid cell-specific leucine-rich glycoprotein) receptor

The CD14 receptor is a myeloid cell specific receptor, which plays a role i n the recognition of lipopolysaccharides (endotoxins of Gram-negative bacte ria) and cell stimulation. To date, several sequences of the cDNA of the CD 14 receptor have been described. We sought to establish whether the substit utions C(230) --> G, and G(560) --> A are polymorphic or if they result fro m a PCR or sequencing error. Using two mismatched PCRs, we confirmed (on 75 unrelated probands) that the substitutions are not due to common polymorph isms. The common cDNA sequence has the C in position 230 and G in position 560. This corresponds to the amino acids Ala and Cys in positions 77 and 18 7, respectively.