Ai. Bhat et al., Characterization of a potyvirus from eggplant (Solanum melongena) as a strain of potato virus Y by N-terminal serology and sequence relationships, PLANT PATH, 48(5), 1999, pp. 648-654
A potyvirus (eggplant mottle virus, EMoV) causing mosaic mottling in eggpla
nt (Solanum melongena) was characterized on the basis of biological, serolo
gical and partial nucleotide sequence properties. EMoV infected Chenopodium
amaranticolor and members of the Solanaceae. Polyclonal antiserum against
EMoV showed antigenic relationship with henbane mosaic potyvirus (HMV) and
potato Y potyvirus (PW). Virus-specific antibodies directed to the N-termin
al region of EMoV cross-reacted only with PVY. Determination and comparison
of nucleotide sequence of the coat protein (CP) and the 3'-untranslated re
gion (UTR) of EMoV with other potyviruses showed that the level of homology
was highest with PVY isolates. Comparative sequence analyses of the CP ami
no acid and 3'-UTR sequences with distinct PVY isolates placed EMoV within
the PVYO subgroup.