Characterization of a potyvirus from eggplant (Solanum melongena) as a strain of potato virus Y by N-terminal serology and sequence relationships

A potyvirus (eggplant mottle virus, EMoV) causing mosaic mottling in eggpla nt (Solanum melongena) was characterized on the basis of biological, serolo gical and partial nucleotide sequence properties. EMoV infected Chenopodium amaranticolor and members of the Solanaceae. Polyclonal antiserum against EMoV showed antigenic relationship with henbane mosaic potyvirus (HMV) and potato Y potyvirus (PW). Virus-specific antibodies directed to the N-termin al region of EMoV cross-reacted only with PVY. Determination and comparison of nucleotide sequence of the coat protein (CP) and the 3'-untranslated re gion (UTR) of EMoV with other potyviruses showed that the level of homology was highest with PVY isolates. Comparative sequence analyses of the CP ami no acid and 3'-UTR sequences with distinct PVY isolates placed EMoV within the PVYO subgroup.