CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF ESCHERICHIA-COLI PEPTIDYL-TRANSFER-RNA HYDROLASE

Authors
SCHMITT E FROMANT M PLATEAU P MECHULAM Y BLANQUET S
Citation
E. Schmitt et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF ESCHERICHIA-COLI PEPTIDYL-TRANSFER-RNA HYDROLASE, Proteins, 28(1), 1997, pp. 135-136
Citations number
14
Categorie Soggetti
Biology
Journal title
Proteins → ACNP
ISSN journal
08873585
Volume
28
Issue
1
Year of publication
1997
Pages
135 - 136
Database
ISI
SICI code
0887-3585(1997)28:1<135:CAPAOE>2.0.ZU;2-E
Abstract
Peptidyl-tRNA hydrolase from Escherichia coli, a monomer of 21 kDa, wa s overexpressed from its cloned gene pth and crystallized by using pol yethylene glycol as precipitant. The crystals are orthorhombic and hav e unit cell parameters a = 47.24 Angstrom b = 63.59 Angstrom and c = 6 2.57 Angstrom. They belong. to space group P2(1)2(1)2(1) and diffract to better than 1.2 Angstrom resolution. The structure is being solved by multiple isomorphous replacement. (C) 1997 Wiley-Liss, Inc.