Comparison of chimeric and non-chimeric tolerance using posttransplant total lymphoid irradiation - Cytokine expression and chronic rejection

Background. Previous studies showed that an intravenous infusion of donor b lood cells facilitates tolerance to ACI heart allografts in Lewis rat hosts given posttransplant total lymphoid irradiation (TLI) and anti-thymocyte g lobulin (ATG), The object of the current study was to compare tolerance ind uction using donor cells that do or do not induce chimerism, Methods. Normal peripheral blood mononuclear cells (PBMC), granulocyte colo ny-stimulating factor (G-CSF)-mobilized PBMC, and bone marrow (BM) cells fr om ACI donors were tested for their capacity to prolong ACI heart allograft survival in Lewis hosts, Chimerism, anti-donor cell reactivity, and cytoki ne gene expression in grafts were determined. Results. Intravenous injections of equal numbers of all three donor cells m arkedly prolonged graft survival (median: >164 to >175 days) as compared to uninjected controls (median: 53 days). Chimerism among T and B cells in th e blood was determined by immunofluorescent staining in hosts bearing long- term (>150 days) grafts. Although no chimerism was detected in hosts given normal or G-CSF-mobilized PBMC, chimerism was detected at variable levels i n all hosts given BM cells. Vigorous anti-donor reactivity in the mixed leu kocyte reaction was present only in non-chimeric hosts, Long-term grafts fr om hosts given normal ACI PBMC developed chronic rejection, but those from hosts given ACI BM cells did not. The latter hosts showed the lowest levels of intragraft cytokine mRNA. Conclusions. Chimeric tolerance is more robust than non-chimeric tolerance in the model of posttransplant TLI, ATG, and donor cell infusion, and is as sociated with less chronic rejection.