Automation of functional assays by flow injection fluorescence microscopy

Authors
Scampavia, LD Hodder, PS Lahdesmaki, I Ruzicka, J
Citation
Ld. Scampavia et al., Automation of functional assays by flow injection fluorescence microscopy, TRENDS BIOT, 17(11), 1999, pp. 443-447
Citations number
20
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
TRENDS IN BIOTECHNOLOGY
ISSN journal
01677799 → ACNP
Volume
17
Issue
11
Year of publication
1999
Pages
443 - 447
Database
ISI
SICI code
0167-7799(199911)17:11<443:AOFABF>2.0.ZU;2-S
Abstract
Bead-injection spectroscopy is a novel technique that uses immobilized euka ryotic cells on microbeads as a renewable biosensor for fluorescence micros copy. The use of a flow injection instrument allows fast functional assays that generate full kinetic characterization of a drug. Because the cell pop ulation is automatically replaced for each assay, Variability is minimized, thus allowing greater accuracy.