Lipid composition of subcellular membranes of an FY1679-derived haploid yeast wild-type strain grown on different carbon sources

The aim of the project EUROFAN (European Functional Analysis Network) is to elucidate the function of unknown genes of the yeast Saccharomyces cerevis iae at a large scale. Functional analysis is based on general and specific tests with yeast deletion strains. A prerequisite for these studies is a pr ofound knowledge of the biochemistry and cell biology of the corresponding wild-type strain FY1679. As a contribution from our laboratory we present h ere a systematic lipid analysis of the major organelles isolated from FY167 9 grown in the presence of different carbon sources. Phospholipid, sterol a nd fatty acid composition are characteristic for each organelle. Moreover, growth of the yeast on glucose, ethanol or lactate causes in some cases mar ked changes of the organelle lipid pattern. As the most prominent example, cultivation of the yeast on non-fermentable carbon sources results in an in crease of mitochondrial cardiolipin. As another example, the ratio of unsat urated to saturated fatty acids is enhanced in cells grown on ethanol or la ctate as compared to glucose. Thus, the lipid composition of yeast subcellu lar membranes reflects in a significant way the nutrient conditions caused by variation of the carbon source. Copyright (C) 1999 John Wiley & Sons, Lt d.