We have applied counting of annual cementum lines in canine root to estimat
e the age of 165 Iberian wildcats Felis silvestris Schreber, 1775. Results
indicate that (1) closure of apical foramen of canine root occurs from 6 to
18 months of age, (2) cementum lines are formed in autumn-winter, (3) the
first autumn-winter, either a non-continuous line, a continuous "kitten lin
e" or no line can be formed, depending on individual variation in timing of
closure of apical foramen, (4) since 7% of wildcats form a kitten line, it
needs to be identified in order to avoid an incorrect counting, (5) the fi
rst continuous line useful for counting is formed during the second autumn-
winter, when animals are 21 months old. For Iberian wildcats, age in years
can be estimated by adding 1 to the number of continuous lines. For a more
accurate estimation, we propose a formula including months from birth to fi
rst line formation, number of annual lines, and months from last line forma
tion to death (A = 9m + Ly + Mm).