S. Toppila et al., Endothelial L-selectin ligands are likely to recruit lymphocytes into rejecting human heart transplants, AM J PATH, 155(4), 1999, pp. 1303-1310
Citations number
46
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
L-selectin-dependent lymphocyte extravasation is a hallmark of acute heart
allograft rejection in rats. On screening over 600 endomyocardial biopsies
(EMBs), taken at different time points after heart transplantation in man,
we identified 91 samples with histological signs of acute rejection. Reject
ion and nonrejection EMBs were analyzed for the presence of properly glycos
ylated, ie, sulfated sialyl Lewis-x (sLex) decorated L-selectin ligands, Tw
o anti-sLex (2F3 and HECA-452) and one anti-6- or 6'-sulfated and/or 6,6'-b
isulfation (MECA-79) monoclonal antibodies were used. Nonrejecting heart en
dothelium did not express, or expressed only weakly, sulfated and or sLex d
ecorations of L-selectin ligands. On the contrary, these epitopes were read
ily detectable on endothelium of capillaries and venules at the onset and d
uring acute rejection episodes. The more intense the sulfated sLex expressi
on was, the more severe the rejection episode was in histological grading.
The endothelial expression of L-selectin ligands decreased to background le
vels as the rejection resolved, Our data demonstrate a complete correlation
between the level of expression of the sulfated sLex-decorated ligands on
the one hand and the histological severity of acute heart allograft rejecti
on on the other hand. These data suggest that functionally active endotheli
al L-selectin ligands are instrumental in lymphocyte extravasation into the
human heart allografts at the onset and during acute rejection episodes.