Differentiation of normal and neoplastic cells by synchronous fluorescence: Rat liver epithelial and rat hepatoma cell models

In the present study, conventional and synchronous luminescence (SL) were u tilized to investigate spectral differences in normal and neoplastic cells. The synchronous fluorescence (SF) method involves scanning simultaneously both emission and excitation wavelengths while keeping a constant wavelengt h interval between them. This SF procedure simplifies the emission spectrum and provides for greater selectivity and is used to detect subtle differen ces in the fluorescence emission of the biochemical species of cells from r at tissues. A difference between the fluorescent spectra of the normal rat liver epithelial (RLE) and hepatoma cell lines were detected using synchron ous fluorescence. The potential use of SF as a screening tool for cancer di agnosis is discussed.