QUANTITATIVE-DETERMINATION OF THE COMPONENTS IN OVERLAPPING CHROMATOGRAPHIC PEAKS USING WAVELET TRANSFORM

A novel application af the wavelet transform in retrieving the separat e signals from overlapping chromatographic peaks and quantitative dete rmination of the components in the overlapping chromatograms is descri bed, The signals can be very easily separated by decomposing an overla pping chromatographic peak into localized contributions according to t heir frequency, and quantitative calculation can be done by studying t he contributions of higher frequency. Overlapping peaks of two- and th ree-component mixtures were investigated by the method, and the result s slow excellent correlations between peak areas of the retrieved sign als and the concentrations for all of the components.