Synthesis and fluorescence properties of Oregon Green 514 labeled peptides

Authors
Delmotte, C Delmas, A
Citation
C. Delmotte et A. Delmas, Synthesis and fluorescence properties of Oregon Green 514 labeled peptides, BIOORG MED, 9(20), 1999, pp. 2989-2994
Citations number
13
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
9
Issue
20
Year of publication
1999
Pages
2989 - 2994
Database
ISI
SICI code
0960-894X(19991018)9:20<2989:SAFPOO>2.0.ZU;2-G
Abstract
Oregon Green 514, with high photostability and a pKa of 4.8, is suitable fo r imaging applications. Labeling of peptide-bound resin with the carboxylic acid form was optimized. Peptide conjugation resulted in altered fluoresce nce properties of the dye including a quenching of the intensity compared w ith that in aqueous buffers with a slight red-shift of the emission maximum . (C) 1999 Elsevier Science Ltd. All rights reserved.