Performance comparison of Protein A affinity-chromatography sorbents for purifying recombinant monoclonal antibodies

We describe the performance characteristics of five Protein A affinity-chro matography sorbents (Sepharose Fast Flow, Poros 50, Poros LP, Prosep and St reamline) for purifying a recombinant humanized monoclonal antibody from cl arified Chinese hamster ovary cell culture fluid. We measured the dynamic c apacity at varying flow rates, maximum capacity, pressure drop and producti on rate, For purified antibody, we measured yield and purity (by SDS/PAGE, the amount of DNA, the amount of host-cell proteins and the amount of Prote in A). We found that, whereas all sorbents provided significant and essenti ally equivalent antibody purification, there were differences in capacity a nd pressure drop, which affected the production rate and had implications f or process applications.