Pyridoxine as a substrate for screening synthetic potential of glycosidases

The reactions of glycosidases with pyridoxine were used for testing their a bility to make new glycosidic bonds. Of 35 glycosidases examined, some exhi bited regiospecificity towards one primary alcoholic group; glycosylation o f phenolic hydroxyl group was not observed. A series of new glycosides of p yridoxine, 2-acetamido-2-deoxy-beta-D-glucopyranosides alpha-D-manno-pyrano sides, and one alpha-D-galactopyranoside were prepared and completely chara cterized by MS and NMR.