Toxicity of conventional, elemental chlorine-free, and totally chlorine-free kraft-pulp bleaching effluents assessed by short-term lethal and sublethal bioassays

The toxicity of nine effluents from Swedish kraft-pulp mills using conventi onal, elemental chlorine-free (ECF) and totally chlorine-free (TCF) bleachi ng processes was studied. Effluents represented six different processes: co nventional-softwood 30% ClO2; ECF-Softwood; TCF-hardwood, for which all the samples were taken before and after secondary treatment; conventional-soft wood 8% ClO2,; TCF-softwood O-3/H2O2; and TCF-softwood H2O2. Comparison of effluents, which had not undergone secondary treatment, suggested that thos e from mills using conventional bleached pulp were the most toxic, with tox ic emission factors (TEF) approximate to 1,000 according to the Microtox(R) test (AZUR Environmental, Carlsbad, CA, USA). In the acute Nitocra spinipe s test, the conventional-softwood 8% ClO2 and the ECF-softwood effluents be fore secondary treatment were the most toxic, with TEF approximate to 700. In the reproduction test, N. spinipes was two to three times more sensitive than in the lethal test. The species most sensitive to the effluents inves tigated was the macroalga Ceramium strictum, with TEF values up to 3,000. T he conventional effluent using a substitution of 8% ClO2 was the most toxic , and the TCF effluents were the least toxic. Degradation treatment of the ECF and TCF effluents for 28 d in the laboratory did not eliminate acute le thal effects on N. spinipes and the Microtox bacterium.