A reproducible protocol for the production of gynogenic haploids of mulberry, Morus alba L.

Gynogenic haploids of a female clone of mulberry ( Morus alba L. Cv. K-2) w ere produced by unpollinated ovary culture. Segments from in vitro develope d inflorescences were cultured on MS+ BAP (8.5 mu M)+2,4-D (4.5 mu M) and a fter three weeks individual ovaries were transferred to MS+2,4-D (4.5 mu m) +glycine (6660 mu M)+proline (1738 mu M). In this treatment sixteen per cen t of the ovaries developed a gynogenic seedling. The gynogenic plants have been established in soil. Out of twenty gynogenic plants examined cytologic ally, twelve showed the haploid number of chromosomes (2n=x=14) and the oth er eight were aneuploids, with 13-17 chromosomes in their root tip cells.