Comparison of high-performance liquid chromatography and capillary electrophoresis methods for quantitative determination of glycyrrhizinic acid in pharmaceutical preparations
Hk. Hansen et al., Comparison of high-performance liquid chromatography and capillary electrophoresis methods for quantitative determination of glycyrrhizinic acid in pharmaceutical preparations, EUR J PH SC, 9(1), 1999, pp. 41-46
A high-performance liquid chromatographic (HPLC) and a capillary electropho
retic (CE) method have been developed for the determination of glycyrrhizin
ic acid in pharmaceuticals. The two methods have been validated and have be
en compared with respect to their suitability for the said purpose as well
as in relation to requirements from the legal authorities. The HPLC method
provide a repeatability of the quantitative analysis of glycyrrhizinic acid
below 1% relative standard deviation (RSD) which makes the method suitable
when the legal authorities requires the content to be within +/-5% of the
declaration. The repeatability of the CE method is in the order of 3-5% RSD
when using internal standardization. However, using internal standardizati
on as well as peak normalisation (peak area/migration time) indicate that t
he RSD may be reduced to about 1%. The CE method is suitable as a stability
indication method as the degradation product glycyrrhetinic acid can be de
termined simultaneously. (C) 1999 Elsevier Science B.V. All rights reserved
.