alpha-adrenoceptor-mediated pressor responses in pithed rats fed diets with different calcium contents

Results of many clinical and experimental studies indicate an inverse relat ionship between dietary calcium and the prevalence of hypertension. Our stu dy was designed to evaluate the alterations in arterial blood pressure and the changes in a-adrenoceptor-mediated vascular reactivity in normotensive Sprague-Dawley and spontaneously hypertensive rats (SHR) fed from weaning ( 3 weeks of life) three diets: normal calcium (Ca 1%), low calcium (Ca 0.1%) , and high calcium (Ca 2.5%). The systolic and the diastolic arterial blood pressures were measured weekly by the tail cuff method. The plasma calcium levels in the animals were also measured regularly by colourimetric method s, and the alpha-adrenoceptor-mediated vascular reactivity was evaluated by measuring the presser responses to a-adrenoceptor agonists in pithed rats. These determinations were carried out at the end of the feeding periods (9 weeks of life in Sprague-Dawley rats and 20 weeks of life in SHR) and also at the moments when maximal differences in arterial blood pressure were ob served between the conscious animals fed the normal calcium diet and those fed the other two diets. Dietary calcium deficiency increased arterial bloo d pressure in both strains but calcium supplements were effective to lower this only in hypertensive animals. The plasma calcium levels were altered i n both strains when calcium administration was not normal. The low-calcium diet did not modify the presser responses to either the alpha(1)-adrenocept or agonist, methoxamine, or the alpha(2)-adrenoceptor agonist, B-HT 920 (5- allyl-2-amino-5,6,7,8-tetrahydro-4 H-thiazolo-(4,5-D)-acepin-dihydrochlorid e talixepole), in the normotensive and the hypertensive rats. On the contra ry, the high-calcium diet caused a definite decrease in alpha(1)- and alpha (2),-adrenoceptor-mediated vascular reactivity in both strains. The changes in the a-adrenoceptor-mediated vasoconstrictor responses were observed in pithed 9-week old Sprague-Dawley rats and in pithed 20-week old SHR, but no ne were observed in pithed 15-week old SHR, although at this age maximal di fferences in arterial blood pressure between the animals fed the high- and the normal calcium diet were observed. The results of this study suggest th at the mechanisms implicated in the effects of dietary calcium supplements on arterial blood pressure are clearly different from the mechanisms, which bring about changes in arterial blood pressure when the diet is deficient in calcium. The results of this study also show that calcium administration causes variations in alpha-adrenoceptor-mediated vascular reactivity, but this is probably not the only mechanism implicated in the calcium effect on arterial blood pressure. (C) 1999 Elsevier Science B.V. All rights reserve d.