Two 13.1 m(2) ponds at Auchincruive, Scotland, were used to treat the dilut
ed liquid phase of separated piggery slurry, in order to identify the clima
tic and pond operational parameters which influence biomass production and
nutrient removal in these systems at a constant areal loading rate. The pon
ds were operated from April to November at 0.12, 0.24 and 0.34 m depth as b
atch-fed reactors. Average 5-day biochemical oxygen demand (BOD5) loading w
as 6.24 g m(-2) d(-1) and the ponds were mixed at a mean surface velocity o
f 0.20 m s(-1). Dry matter, chlorophyll, optical density (OD560), NO3, NO2,
NH4, urea and total phosphorus were determined daily. Temperature, pH, dis
solved oxygen and incident irradiance were monitored continuously. Correlat
ion and multiple regression analyses were used to determine significant int
eractions between environmental factors, biomass production and nutrient re
moval. Both chlorophyll a and optical density were accurate predictors of d
ry matter biomass, All measures of pond biomass were positively correlated
with elapsed time, surface daily irradiance, daylength and pH, but negative
ly correlated with pond depth. Significant correlations between pH and dail
y irradiance, maximum dissolved oxygen and forms of nitrogen (nitrite or ni
trate) suggested that the final pond pH represents an equilibrium between a
lkalization by photosynthesis and acidification by nitrification. Total nit
rogen removal was influenced by biomass, elapsed time, temperature and dail
y irradiance, but not by either pH or depth. The concentration of ammonium
nitrogen (NH4-N) was inversely correlated with temperature, biomass, depth,
daily irradiance and daylength. Nitrification was found to occur with nitr
ate concentration showing a strong negative correlation with daylength, ref
lecting an increase in nitrifying activity by the pond biomass throughout t
he season. Nitrate concentrations were positively correlated with elapsed t
ime, but negatively correlated with biomass, temperature and daily irradian
ce. Phosphorus removal was influenced by elapsed time and biomass concentra
tion. Removal of biological and chemical oxygen demand (COD) at the complet
ion of the batch runs was 96% and 78.6% respectively.