Jn. Mcdougal et al., INFLAMMATORY DAMAGE TO SKIN BY PROLONGED CONTACT WITH 1,2-DICHLOROBENZENE AND CHLOROPENTAFLUOROBENZENE, Microscopy research and technique, 37(3), 1997, pp. 214-220
Skin samples from Fischer-344 rats and Hartley guinea pigs exposed der
mally to 1,2-dichlorobenzene (DCB) and chloropentafluorobenzene (CPFB)
for up to 4 h were examined for chemical-induced damage. Samples were
stained with hematoxylin and eosin and scored for polymorphonuclear c
ell (PMN) margination, dermal inflammation, and epidermal necrosis by
light microscopy. Ultrastructural evaluation of samples fixed with 2%
glutaraldehyde and postfixed with 1% osmium tetroxide was used to visu
alize margination of PMNs. Guinea pigs exhibited postexposure inflamma
tory changes following an exposure of about an hour-and-a-half shorter
duration than rats. DCB-induced inflammation and PMN margination occu
rred following an exposure about a half hour shorter in both species c
ompared to CPFB. In contrast, epidermal necrosis was more severe with
CPFB than with DCB. These changes may account for decreases in chemica
l penetration rates which have been observed in previous studies with
DCB and CPFB in rats and guinea pigs. (C) Wiley-Liss, Inc.